Dynamic Biosensors helix cyto Normalization Solution
Specifications
- Navê Berhem: heliX cyto Normalization Solution (Red Dye)
- Hejmara Siparîşê: NOR-R2
- For scIC measurements in the red channel
- Tenê ji bo lêkolînê bikar bînin
- Limited shelf life – please check expiry date on label
Taybetmendiyên sereke
- Ji bo normalîzekirina sînyalên fluorescentê yên li ser Xala 1 û Xala 2 ya çîpek heliXcyto
- Enables correct real-time referencing of the red fluorescent signals during scIC measurements
- Compatible with all heliXcyto chips
- The Normalization solution (red dye) contains a hydrophilic red dye with a single negative net charge.
Danasîna hilberê
- Hejmara Siparîşê: NOR-R2
Table 1. Naverok û Agahdariya Storage
| Mal | Devik | Concentr ation | Biha | Embarkirinî |
| Normalization solution-R2 | Sor | 10 μM | 6x 100 μL | -20°C |
- Tenê ji bo lêkolînê bikar bînin.
- This product has a limited shelf life; please see the expiry date on the label.
- To avoid many freeze-thaw cycles, please aliquot the solution.
Amadekarî
- Use this red dye normalization solution for scIC measurements in the red channel (analyte label-dependent).
- Dilute the 10 µM normalization stock solution to a working concentration with running buffer.
- The concentration of the normalization solution should approximately correspond to the fluorophore concentration in the highest analyte concentration to be measured. This can be calculated usingthe following equation:
Concentration of the normalization solution in the desired color :
Di çareya analîtê ya nîşankirî de kombûna boyaxkirinê
Highest concentration of the analyte that should be measured
Degree of labeling (ratio of dye to analyte)
Çareseriyên hûrkirî dikarin di 2-8 ° C de heya 7 rojan bêne hilanîn.
Têbînî Serlêdan
In the scIC measurement, the fluorescent signal of the normalization solution should be in a similar range to the highest signal coming from the bound analyte (raw data). The absolute fluorescent signal is dependent on normalization solution concentration and the excitation power applied in the measurement. The excitation power has to be selected based on the following parameters:
- Fluorophore concentration inthe analyte solution
Giraniya fluorophore bi giraniya analîtê ya ku di pîvandinê de tê bikar anîn û her weha asta nîşankirina analîtê ve girêdayî ye. Ji bo DOL-ya bilind û giraniya analîtê ya bilind, dibe ku kêmkirina hêza heyecanê hewce bike. - Sinyala girêdanê ya hêvîkirî
Highly expressed targets on a cell can bind more molecules of the labeled analyte. In case of highly overexpressed targets, a strong binding signal can be expected. To avoid the shutter closing, lowering the excitation power might be considered. - Cureyê çîpê
Cûreyên çîpê yên cihêreng xwedan paşnavên fluorescent ên cihêreng in. The xefik mezintir û bêtir xefikên li ser çîpê, bilindtir sînyala paşperdeya. Ji ber vê yekê, çîpên L5 dibe ku ji çîpên M5-ê re hêza heyecanê kêmtir hewce bike.
For a starting point of excitation power and normSolution on concentration to be used in an scIC experiment, please refer to Table 2.
Table 2. Relation of fluorophore concentration, normalization solution concentration, and excitation power suitable for an 
çîpê M5
| Analit dye conc. = analyte conc x DOL | Hêza heyecanê | Çareseriya Normalîzasyona Kêmkirinê | Çareseriya Normalîzasyona Dilution |
| 25 nM | 0.5 | 25 nM | 1:400 |
| 50 nM | 0.3 | 50 nM | 1:200 |
| 100 nM | 0.2 | 100 nM | 1:100 |
| 300 nM | 0.1 | 300 nM | 1:33 |
| 500 nM | 0.08 | 500 nM | 1:20 |
| 1 μM | 0.05 | 1 μM | 1:10 |
| 2.5 μM | 0.02 | 2.5 μM | 1:4 |
Not: This table is for your guidance. However, the final signal recorded in the heliXcyto depends on many factors. Thus, some optimization will be required for each system.
Têkelî
Dynamic Biosensors GmbH
Perchtinger Str. 8/10 81379 Munich, Germany
Bruker Scientific LLC
40 Manning Road, Manning Park, Billerica, MA 01821 DYA
- Order Information
- Piştgiriya Teknîkî
www.dynamic-biosensors.com
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FAQs
What is the shelf life of the heliX cyto Normalization Solution?
Hilber xwedan jiyanek bisînor e. Ji kerema xwe ji bo agahdariya taybetî dîroka qedandina li ser labelê kontrol bikin.
How should I store the NOR-R2 v1.0 product?
The product should be stored according to the storage information provided in Table 1 of the user manual.
Can I use this product for clinical purposes?
No, this product is for research use only.
Belge / Çavkanî
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Dynamic Biosensors helix cyto Normalization Solution [pdf] Rêbernameya Bikarhêner helix cyto Normalization Solution, helix cyto, Normalization Solution, Solution |